Reliable Quantitation of Gluten

AgraQuant® Gluten G12

Quantitation of Gluten Residue in Foods, Surfaces and Rinse Waters

Dietary gluten present in barley, oats, rye and wheat are responsible for the immune mediated condition known as Coeliac disease. The G12 antibody has been used by Romer Laboratories to develop the AgraQuant® Gluten G12 and the AgraStrip® Gluten G12 kits. The incorporation of the G12 antibody offers a more reliable test, as it targets the toxic fragment that riggers the auto immune reaction in coeliac patients – the 33mer peptide found in the alpha-2 gliadin protein. The G12 antibody can detect potential immune-toxic varieties of oats. The AgraQuant® Gluten G12 test kit offers food manufacturers a tool to quantitate Gluten residue and prevent the release of contaminated product.

The AgraQuant® Gluten G12 kit is a sandwich enzyme-linked immunosorbent assay (ELISA) that quantitates the level of specific immunotoxic gluten fragments in a variety of foods, environmental surfaces and rinse waters. The kit is supplied with standards, antibody coated microwells, extraction buffer, wash buffer, conjugate, substrate and stop solution.

  • Sensitive – Quantitates low levels of Gluten residue
  • Rapid – 60-minute incubation time
  • User friendly – ready to use reagents & simple handling procedures across the AgraQuant® range
  • Save on costs – breakaway wells, only use the number of wells required
  • Long shelf life – 12 months from date of manufacture
  • AOAC Official Method (OMA) 2014.03


Specifications: -

  • 96 wells
  • Limit of Detection: 2ppm Gluten
  • Limit of Quantitation: 4ppm Gluten
  • Range of Quantitation: 4-200ppm
  • No cross reactivity to: Pecan, Walnut, Almond, Cashew, Macadamia, Peanut, Hazelnut, Pine Nuts, Pistachio, Linseed, Poppy Seed, Sesame, Mustard, Soya, Millet, Buckwheat, Quinoa, Fennel, Chickpea, Coriander, Black Eyed Beans, Puy Lentils, Cardamom.


Video Demonstration
AgraQuant® Allergen Test Kit Methodology

AgraQuant® Gluten G12 Brochure
AgraQuant® Allergen Brochure
AgraQuant® Product Focus Brochure
AgraQuant® Allergen Swabbing Kit
Product Focus – Gluten Analysis. Next generation Gluten Detection Technology

Quick Guide
AgraQuant® Quick Guide

Pack Insert
AgraQuant® Gluten G12 Pack Insert
AgraQuant® Allergen Swabbing Kit

Technical Documents
AgraQuant® Validation Summary
AgraQuant® Gluten G12 Validation Report

Application Notes
Procedure for the Analysis of Rinse Waters Using AgraQuant® Allergen Test Kits

Ordering Details
AgraQuant® ELISA Gluten G12: 4-200ppm (LOD 1.4ppm)
Product Code: RL1001994
Number of wells: 96



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