DETECTION OF C. DIFFICILE TOXIN B FROM HUMAN FECAL SPECIMENS
The C. DIFFICILE TOX-B TEST uses a tissue culture format to detect the presence of cytotoxic activity (cell rounding) in fresh or frozen human fecal specimens and confirms the identification of C. difficile toxin by using specific antitoxin.
The reagents in the C. DIFFICILE TOX-B TEST kit include toxin control, specific antitoxin, Phosphate Buffered Saline, and diluent. The cytotoxic effect may be observed within 8 hours after applying the specimen if high amounts of toxin are present. However, specimens should not be considered negative until after 48 hours.
TECHLAB® C. DIFFICILE TOX-B TEST:
Clostridium difficile infection usually occurs when the normal microflora of the intestinal tract is altered or killed by antibiotics. Once toxigenic C. difficile growth begins, toxins are produced, causing diarrhea and colitis. Toxigenic strains of C. difficile carry the genes encoding the toxins. The disease results from the toxins that the organism produces.
C. difficile produces two main toxins, toxin A and toxin B, toxigenic C. difficile strains may produce both toxins or only toxin B. Toxin A is a weakly cytotoxic enterotoxin. C. difficile also produces toxin B, which is a cytotoxin and can be detected by the tissue culture assay.
Product Code: TLI-30399U C. DIFFICILE TOX-B (96 wells)