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Next Day Detection ELISA Method
Description

Solus™ ONE Salmonella

Next Day Detection of Salmonella Species for Production & Environmental Samples

Solus Salmonella Test Kit

A highly efficient assay for the next day detection of Salmonella spp. in environmental and product samples. Solus ONE Salmonella provides a negative or a presumptive positivie result from a single enrichment step in under 19 to 25 hours.

Solus ONE Salmonella is the newest addition to the Solus Scientific is the newest addition to the Solus Scientific pathogen systems range, which comprise of specifically designed immunoassay kits, dedicated selective enrichment media and automated liquid handling.

Solus ONE Salmonella is the newest addition to the Solus Scientific pathogen systems range, which comprise of specifically designed immunoassay kits, dedicated selective enrichment media and automated liquid handling. 

Solus ONE Salmonella uses a single enrichment media, Buffered Peptone Water supplemented with Solus ONE Salmonella supplement. Once reconstitute the supplement and media is stable for 2 weeks refrigerated. 

Faster time to result

The Solus ONE range of kits utilised enhance ELISA technology and larger microplate wells to increase the available antigen binding sites. This decreases assay limit of detection and reduces enrichment times. In a fast-paced food testing environment it is critical to process samples quickly and efficiently, enabling the production facility to take prompt corrective actions when necessary. 

If Salmonella is detected the lab must have the flexibility to cope with increasing sample vaolumes while extensive investigative sampling is being carried out, maximising throughput without the need for additional resources. 

  • Next generation, enhanced ELISA technology - detects as low as 104 cfu / mL
  • Next day results - 16-22hr enrichment + 2hr 15 min assay run
  • Single enrichment - Buffer Peptone Water with supplement
  • Less hands on time - 1 technician can run 2 DS2 instruments = 742 tests per 8hr shift
  • Walk away Ds2 automation - only 1 hour hands on time for 2 x 96 tests
  • Flexible and scalabe - copes with changing testing loads
  • Sample traceability when using the on-board bar-code facility
  • Small instruments footprint maximises bench space, compact product packaging reduces cold storage space requirement and packaging waste when compated to other methods
  • AOAC PTMSM Validation - food matirces include raw Salmon, cheese, lettuce, non fat dey milk, stainless steel surface, plastic surface, liquid egg

Sample Enrichment & Processing

Buffered Peptone Water should be pre-warmed to 37°C prior to sample inoculation. Enrichment times are as follows;

  • Environmental samples - 100mL for sponges, 10mL for swabs - incubate 16-20hrs, 41.5°C
  • Food samples - 25g sample into 225mL of BPW - incubate 20-22hours, 41.5°C
  • 100g samples (egg) - add 900mL of BPW - incubate 20-22hrs, 41.5°C
  • 375g samples (meat) - add 1125mL (1 in 4 dilution) - incubate 20-22hrs, 41.5°C 

Enrichment is followed by a heat kill step - 85-100°C for 15-20min. The samples are ready to be run on the Solus ONE Salmonella ELISA kit. 

 Solus One Salmonella M1Solus One Salmonella M2

Brochure

Instructions

Ordering Details: -

Product Code Description Pack Size
SS-SAL1-0096 Solus ONE Salmonella ELISA 96
SS-SAL1-0480 Solus ONE Salmonella ELISA 480
SS-SALSUPP-22.5 Solus ONE Salmonella Supplement 22.5L 1
SS-SALSUPP-112.5 Solus ONE Salmonella Supplement 112.5L 1

 

 

 

 

 

 

 

 

 

 

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