Microgen E.coli 0157 is an easy-to-use one step, latex identification kit which gives results in under two minutes and specifically identifies E.coli 0157 directly from the selective plate, even in mixed culture.
The conventional method for identification of E. coli 0157:H7 utilises specific antisera in a standard agglutination test. However, this method and other commercially available latex tests have been shown to suffer from cross reactions with other enteric organisms such as E. hermanii, which antigenically and biochemically resembles E.coli 0157, resulting in false positive results. MicrogenE. coli 0157 contains a test latex which has been sensitised with E. coli 0157 specific antibodies, that are prepared by absorption with potentially cross reacting strains.
Microgen E. coli 0157 has demonstrated no cross reactions when tested against a panel of 161 E. coli 0157 ‘O’ serotypes and 56 ‘H’ serotypes, and shows strong specific agglutination.
Microgen E. coli 0157 gives strong and specific results within two minutes and comes complete with a control latex which can be easily compared with test results to confirm non-specific reactions.