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ID disc for Peptostreptococcus, Gardnerella, Haemophilus
Description

HardyDisk™ SPS Identification Disks evaluate the ability of an organism to grow in the presence of Sodium Polyanethol Sulfonate. HardyDisk™ SPS Identification Disks can be used to presumptively identify microorganisms including but not limited to Peptostreptococcus anaerobius, Gardnerella vaginalis, and Haemophilus ducreyi.

SUMMARY

Peptostreptococcus belong to the genus of gram-positive anaerobic cocci. These organisms are widely distributed as normal flora in humans and animals and account for up to a third of anaerobic human isolates.(6) The three species of Peptostreptococcus most commonly reported in clinical specimens are P. anaerobius, P. magnus, and P. asaccharolyticus. Specific identification of most anaerobic cocci requires both biochemical tests and gas-liquid chromatography, as these organisms tend to exhibit few visual phenotypic differences.In contrast to traditional testing procedures, the Sodium Polyanethol Sulfonate (SPS) disk test is a simple and rapid technique used for the presumptive identification of P. anaerobius from other gram-positive anaerobic cocci. The characteristic inhibition by SPS to differentiate P. anaerobius from other gram-positive anaerobic cocci was initially demonstrated by Graves, et al. The SPS disk test has since been demonstrated to have an overall accuracy of 98% in the identification of P. anaerobius from clinical specimens. Consequently, it is recommended that the SPS disk test be routinely applied to all gram-positive anaerobic cocci isolates to presumptively identify P. anaerobius.

SPS inhibition is also useful in the presumptive identification of microorganisms other than P. anaerobius. Reimer and Reller initially demonstrated that SPS disk susceptibility and alpha-hemolytic streptococci inhibition can be employed to accurately identify Gardnerella vaginalis. Currently the differentiation of G. vaginalis from other catalase-negative, gram-variable, coccobacilli is achieved solely on the susceptibility of G. vaginalis to SPS. An additional study performed by Shawar, et al. confirmed that SPS susceptibility can also be used to differentiate Haemophilus ducreyi from other Haemophilus species.  

PRECAUTIONS

For in vitro diagnostic use only. Observe approved biohazard precautions and aseptic techniques. To be used only by adequately trained and qualified laboratory personnel. Sterilize all biohazard waste before disposal.Refer to the keyword "Precautions", in the Hardy Diagnostics software program HUGO™, for more information regarding general precautions when using culture media.Refer to the keyword "MSDS", in the Hardy Diagnostics software program HUGO™, for more information on handling potentially hazardous material.

PROCEDURE

1. Allow disks to equilibrate to room temperature before use. Prepare a suspension, equivalent to a 0.5 McFarland turbidity standard, of the organism to be tested from a pure 24-48 hour culture.

2. Dip a sterile non-toxic swab into the organism suspension. Rotate the swab several times, pressing firmly on the inside wall of the tube above the fluid level. This will remove excess inoculum from the swab. Evenly inoculate the dried surface of a Brucella Agar with Vitamin K and Hemin plate (Cat. no. HDA30) to obtain confluent growth.

3. Place one SPS disk on the media surface. With sterile forceps, gently tap the disk to ensure complete contact with the plate surface.

4. Incubate the media at 35 degrees C. for 24-48 hours. Consult listed references for recommended incubation atmosphere.

5. After incubation, examine the media surface and measure the zone diameter surrounding the SPS disk. Zones should be recorded to the nearest whole millimeter.

INTERPRETATION OF RESULTS

When the zone diameter is greater than or equal to 12mm, the organism is considered susceptible to SPS. When the zone diameter is less than or equal to 11mm, the organism is considered resistant to SPS. 

 

Cat No. HDZ7381

Size: 50 discs

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