For the Detection of Extended Spectrum Beta-Lactamase
HardyCHROM ESBL is a selective chromagenic medium for the primary screening and differentiation of extended-spectrum Beta-Lactamase in Entrobacteriaceae (ESBL). The selective components are agents that inhibit the growth of yeast, gram-positive bacteria and gram-negative extended-spectrum beta-lactam-sensitive bacteria (eg, a cephalosporin). The presences of chromagens allows the differentiation of Enterobacteriaceae that producte ESBL. The colones of those bacteria release products when they use the chromagens as a substrate source appear coloured.
Eschericia coli produces colonies that are rose to magenta in colour, with darker pink centers
Klebsiella and Enterobacter spp. produce large, dark blue colonies
Citrobacter spp. produce dark blue colonies often with a rose halo in the surrounding media
Proteus and Morganells spp. produce clear to light yellow colonies with golden-orange halo diffuesed through surrounding media.