Hardy Diagnostics India Ink is recommended for use in the identification of Cryptococcus neoformans.
Cryptococcus neoformans, because of its large polysaccharide capsule, can be visualized by the India stain. Organisms that possess a polysaccharide capsule exhibit a halo around the cell against the black background created by the India.
Specimen Collection: Specimens should be collected in sterile containers or with sterile swabs and transported immediately to the laboratory. This product is used in conjunction with other biochemical and serological tests to identify cultures of isolated organisms.
Method of Use:
Mix the specimen with a small drop of India on a clean glass slide. Place a cover slip over the smear and press gently. The preparation should be brownish, not black. Using reduced examine the smear microscopically (100X) for the presence of encapsulated cells as indicated by clear zones surrounding the cells.
Note: The India is ready to use. Further dilution with water is not recommended.
Note: Production of capsular material may be increased by cultivation in a 1% peptone solution (Peptone Broth, Cat. no. K151).
Cat. no. HDZ64