The Microgen Bioproducts Avian Immunodiffusion System is a double gel diffusion assay intended for the detection of precipitating antibodies in human serum elicited in response to the inhalation of avian allergens. The diseases associated with the presence of these antibodies have been known variously as pigeon (or budgerigar or bird) handler's (or fancier's or breeder's) lung (or disease) depending on the source of exposure as well as the nomenclature adopted by the investigators. Turkeys, chickens and pheasants can also produce a similar condition and the term hypersensitivity lung disease to pigeons (or other avians) has been used. All these diseases form part of a group of diseases termed extrinsic allergic alveolitis.
The kit is intended for professional laboratory use only.
PRINCIPLE OF THE TEST
The precipitating antibodies detected by the kit are generated as part of the immune response to exposure to antigens derived from pigeons, budgerigars and poultry. These antibodies are predominantly IgG class, although other classes may have a precipitating function.
The test relies on the principle of double gel diffusion (Ouchterlony). When soluble antigens and homologous antibodies are placed in adjacent wells cut into suitable diffusion media such as agar or agarose, they diffuse towards one another and produce visible precipitation lines along the interface of optimal relative concentrations. A multivalent positive control antiserum containing suitable titres of the relevant antibodies can be tested in wells adjacent to the antigens being reacted with the patient's serum. The development of precipitin lines in control tests is used to confirm reagent and plate performance. With experience, the control can be used as a marker to indicate completion of diffusion. By using avian serum and faecal extracts, it is believed that the maximum number of all positive sera will be identified. Consequently, the diagnosis of hypersensitivity reactions should be made considerably easier.
Size: 20 Tests