Clean-Trace Surface Protein Plus Test
RAPID CLEANING VALIDATION SWAB

- Detects protein and reducing agents on surfaces and in hard to reach areas
- Fast results allow immediate corrective action
- Visual reading of a colour change indicates the level of cleanliness
- Detects as little as 50µg of protein
- No instrumentation required
- Easy to use
Neogen Clean-Trace Surface Protein Plus is a rapid, simple and reliable solution to monitor biological contamination on surfaces in real time, helping you to manage your cleaning and protect your customers. Just swab your surface, activate the swab and read the reaction.
Monitoring the effectiveness of your cleaning helps to reduce the risk of a food safety incident—food residues indicate poor cleaning and can lead to bacterial growth.
Instructions For Use

- Place the Neogen Clean‑Trace Surface Protein Plus Test at room temperature for at least 10 minutes before use. Grip the sample stick handle and remove the swab from the device. On dry surfaces use the moisturizer provided and apply 4 drops to the swab OR apply 4 drops to the surface to be tested [figure 1]. Alternatively, the swab can be moistened by placing under a running tap of clean potable water.
- Swab the test area. Where practical, swab an area approximately 10 cm by 10 cm and swab the area in one direction and then swab the area in the opposite direction [figure 2]. Apply pressure on the swab and rotate the swab as the sample is collected to ensure repeatable and effective sampling [figure 3].
- Reinsert the sample stick into the test with the handle inserted to the original position of the unused test. At this point you can either activate and read immediately, or, if it is more convenient, the Neogen Clean‑Trace Surface Protein Plus Test may be labeled and left un-activated for up to four hours before activation and reading of multiple swabs simultaneously.
- To process the sample, push down firmly on the top of the Neogen Clean‑Trace Surface Protein Plus Test sample stick handle [figure 4]. The handle will slide into the device tube and the top of the handle should be level with the top of the device tube when fully depressed. Grip the top of the device and shake rapidly side-to-side for at least five seconds to mix the sample and reagent [figure 5]. The solution will turn mint green.
- Set a timer for 10 minutes and incubate the swabs at room temperature and in a vertical position. After the 10 minute time period, compare the color of the swab and the solution against the color chart on the Neogen Clean‑Trace Surface Protein Plus Test label.
Interpretation


-
Green → Pass
Indicates no detectable protein; surface is considered clean. -
Light Purple → Caution / Low-Level Residue
Suggests minor residual contamination; review cleaning steps. -
Dark Purple → Fail / High-Level Protein Residue
Indicates significant contamination; reclean and re-test surface.
Results should be interpreted within the recommended timeframe to ensure accuracy.
Resources
Clean-Trace Surface Protein Plus Brochure
Neogen Surface Protein Instructions
Ordering Details
Stock Code Stock Code Pack Size
3MPRO50 Neogen PRO50 Surface Protein 50 Test
3MPRO100 Neogen PRO100 Surface Protein 100 Test