QUANTITATIVE ELISA FOR MUSTARD ALLERGENS

 

• Quantitative results in 30 minutes after sample preparation
• High specificity using monoclonal antibodies
• Uniform protocol across the Allergen‑Shield ELISA range
• Broad food matrix compatibility
• Surface swabbing kits available
• Excellent reproducibility and precision

 

For the in‑vitro quantitative determination of mustard residues in food matrices such as dressings, sauces, condiments, meats, mixed herbs, instant soups, ketchup, and spices. Intended for use by trained laboratory personnel in allergen monitoring and quality assurance applications.

 

The Allergen‑Shield Mustard ELISA is a quantitative sandwich enzyme‑linked immunosorbent assay (ELISA) designed for the detection of mustard residues in food products labeled as mustard‑free. The assay provides precise quantification of mustard proteins, ensuring compliance with international allergen labeling regulations (EU No. 1169/2011, FDA, FSANZ, and Codex Alimentarius).

General Information

Mustard (Sinapis alba, Brassica juncea, and Brassica nigra) is a common allergen included in mandatory labeling lists within the European Union and other regulatory jurisdictions. Mustard allergens, including Sin a 1 and Bra j 1, are heat‑stable and resistant to food processing, making sensitive detection essential for protecting consumers.

Principle of the Test

The Allergen‑Shield Mustard ELISA is based on a sandwich enzyme immunoassay. Microtiter plate wells are coated with antibodies specific to mustard proteins. Standards and extracted samples are added to the wells, and if mustard proteins are present, they bind to the immobilized antibodies. After washing, a peroxidase‑conjugated antibody is added to form an antibody‑antigen‑antibody complex. The addition of TMB substrate develops a color proportional to the mustard concentration, and absorbance is read at 450 nm.

 

Performance Characteristics

• Limit of Detection (LOD): 0.25 ppm mustard flour (0.065 ppm mustard protein)
• Limit of Quantification (LOQ): 1.0 ppm mustard flour (0.26 ppm mustard protein)
• Standard curve range: 0–25 ppm
• IC50: 4–12 ppm
• Recovery: 90.8% (CV = 7.5%)
• Intra‑assay CV at 5 ppm: 6.5% (n=16)
• Intra‑assay CV at 15 ppm: 5.4% (n=16)
• The assay exhibits reactivity to yellow (100%), white (50%), brown (75%) and black mustard seed (25%), cabbage seed (41%), rapeseed (12%) and radish seed (0.4%).
• No cross‑reactivity with tree nuts, legumes, or seeds

 

 

Storage and Stability

Store kit and reagents at 2–8 °C. Do not freeze. Protect standards and TMB substrate from light. Reseal unused strips with desiccant immediately after use. Expiry dates are printed on labels.

 

 

Resource

   Allergens Shield Brochure

   Allergen-Shield Mustard ELISA Kit Manual

 

 

Ordering Details

Stock Code        Description                                                                      Pack Size

PGB-A2896           ALLERGEN-SHIELD MUSTARD KIT                                      96

PGB-A2848           ALLERGEN-SHIELD MUSTARD KIT                                     48

PGB-AS1020         ALLERGEN-SHIELD SWABBING KIT                                    20