RAPID CATALASE DETECTION OF BACTERIAL DIFFERENTIATION

INTENDED USE

Hardy Diagnostics Catalase Reagent is useful in the presumptive identification and differentiation of many bacteria. Beta-hemolytic organisms, such as Streptococcus species (catalase-negative), Staphylococcus species (catalase-positive), and Listeria species (catalase-positive) can be differentiated by their catalase reaction.

SUMMARY

Most cytochrome containing organisms produce a catalase enzyme which breaks down hydrogen peroxide into oxygen and water. When a small amount of a catalase producing organism is introduced into hydrogen peroxide, bubbles of oxygen form as a result of the enzyme’s activity.

REAGENT FORMULA

Hydrogen Peroxide, 3%, and Acetophenetidine.

PROCEDURE

Specimen Collection: This product is not intended for primary isolation of patient specimens. This product is used in conjunction with other biochemical tests to identify cultures of isolated organism.Method of Use: With a loop or sterile wooden stick, transfer a small amount of a well isolated, 18 to 24 hour old colony from a non-blood-containing agar onto the surface of a clean, dry glass slide. Immediately place a drop of Catalase Reagent onto a portion of the colony on the slide.

INTERPRETATION OF RESULTS

Observe for the evolution of gas bubbles indicating a positive test.

ORDERING DETAILS

Stock Code: HDZ62 / 15mL

Stock Code:  HDZ76 / 60mL