FOR THE IDENTIFICATION OF STAPHYLOCOCCUS AUREUS

 

• As few as 2 colonies required from primary culture
• Enhanced results with blue carboxylated microparticles (CM-MP)
• Detects all Staphylococcus aureus including MRSA
• Detects Coagulase, Protein A and Capsular Polysaccharide 5 and 8 Antigens
• Definitive results obtained within 20 seconds
• High performance with sensitivity of 100% and specificity of 100%
• Contains negative control
• 100 or 300 Test kit
• Includes new agglutination card design

 

 

The Prolex Staph Xtra Latex Kit provides a rapid method to distinguish staphylococci (particularly Staphylococcus aureus) and methicillin-resistant S. aureus (MRSA) from other species of staphylococci in cultured specimens.

 

The Prolex Staph Xtra Latex Kit utilizes Carboxylate Modified Microparticles (CM-MP) which have been sensitized with fibrinogen and IgG that are specific for capsular types 5 and 8 of Staphylococcus aureus.

 

When Staphylococcal colonies which possess at least one of clumping factor, protein A and / or capsular 5 or 8 antigens are mixed with the latex reagent, the latex particles agglutinate strongly within 20 seconds.

 

All Prolex™ Latex Agglutination Systems now include a specially designed agglutination card with a defined rimmed latex enclosure ring surrounding each circle. This improved design prevents individual test reaction reagents becoming mixed, avoiding compromised results during rocking of the slide.

 

Principle

The Prolex Staph Xtra Latex Kit utilizes polystyrene latex particles which have been sensitized with fibrinogen and IgG that are specific for capsular types 5 and 8 of S. aureus. When Staphylococcal colonies which possess at least one of clumping factor, protein A and/or capsular 5 or 8 antigens are mixed with the latex reagent, the latex particles agglutinate strongly within 20 seconds.

 

Specimen Collection and Preparation of Cultures

A fresh 18–36 hour Gram positive isolate grown on non-selective media such as blood agar should be used.

 

 

Interpretation of Results

Positive: Strong clumping within 20 seconds; negative control shows no reaction.

Negative: No visible agglutination.

Uninterpretable: Agglutination with both reagents indicates autoagglutination.

 

 

Perfomance Characteristics

Kit demonstrated 100% sensitivity and 100% specificity with 50 S. aureus (including capsular 5 & 8 types), 50 MRSA, and 50 MSSA isolates.

 

 

Resources

   Staph Xtra Latex Kit Instructions

   An independent evaluation of the New Prolex® Staph Xtra kit for Staphylococcus aureus

 

 

Ordering Details

Stock Code              Description                                                        Pack Size

PL.1080                      Staphylococcus aureux Xtra Kit                                100

PL.1081                       Staphylococcus aureux Xtra Kit                                300