RAPID DETECTION OF SHIGA TOXIN IN HUMAN FECAL SPECIMENS

The SHIGA TOXIN CHEK™ test is an enzyme immunoassay (ELISA) for the simultaneous qualitative detection of Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2) from human fecal specimens and cultures derived from human fecal specimens.

TECHLAB® SHIGA TOXIN CHEK™

• Results in 30 minutes using the shaking proceedure
• Detects both 0157 and non-0157 strains
• Simple and clear interpretation of results
• LOD is 0.28ng/ml for Stx1 and 0.23 ng/ml for Stx2 for direct fecal specimens

Performance Data compared to Vero Cell Cytotoxin assay:

• In comparative studies for human fecal specimens
  • Sensitivity of 100%
  • Specificity of 9.9%
  • Correlation of 99.9%
• In comparative studies for overnight broth cultures
  • Sensitivity of 97.1%,
  • Specificity of 99.7%
  • Correlation of 99.5%

Shiga toxin-producing Escherichia coli (STEC) cause foodborne and waterborne diarrheal disease that can progress to hemorrhagic colitis and hemolytic uremic syndrome (HUS). STEC derive their name from their ability to produce toxins (Stx1 & Stx2) similar in structure and function to the Shiga toxin produced by Shigella dysenteriae.

STEC strain O157:H7 has historically been the focus of attention in the United States, where it was first isolated from undercooked hamburgers. STEC infections caused by non-O157 strains have become more prevalent in recent years, both in the U.S. and globally. Since certain treatments and medications can increase the risk of HUS, prompt detection of STEC is necessary to prevent outbreaks and secondary transmission.

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Product Code: TLI-T30396                   SHIGA TOXIN QUIK CHEK® (96 wells)